INOGene-Y-Microdeletion RT-PCR Kit
Y chromosome microdeletion is one of the most common genetic disorders associated with male infertility.
Y Chromosome Microdeletions seen in 10-15% of infertile men. It is associated with deletions in chromosome regions called “Azoospermia Factor (AZF)” and divided into 3 parts (AZFa, AZFb, AZFc).
Partial or complete deletions in one or more of these regions cause very low or no sperm production
INOGene Y-Microdeletion Kit includes 8 STS regions, SRY and ZFX/Y regions, including AZF-a, b and c regions.
The INOGene Y-Microdeletion Kit works on the basis of melting curve analysis using SYBR Green dye via Real-time PCR instrument.
Whole blood samples collected in EDTA tubes can be used in RT-PCR analysis after DNA extraction.
The kit components are shown in the table below.
Reagents | Reagents Content | 50 Reactions |
Reaction Mix 1 | SY84, SY134 | 900 µl |
Reaction Mix 2 | SY87, ZFX/Y | 900 µl |
Reaction Mix 3 | SY254, SY127 | 900 µl |
Reaction Mix 4 | SY255, SRY | 900 µl |
Reaction Mix 5 | SY128, SY86 | 900 µl |