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Kit, Reaktif & Sarf Malzemeler » Genetik Hastalıklar » INOGene-Y-Mikrodelesyon RT-PCR Kiti
INOGene-Y-Mikrodelesyon RT-PCR Kiti

 

INOGene-Y-Microdeletion RT-PCR Kit

 

Y chromosome microdeletion is one of the most common genetic disorders associated with male infertility.

Y Chromosome Microdeletions seen in 10-15% of infertile men. It is associated with deletions in chromosome regions called “Azoospermia Factor (AZF)” and divided into 3 parts (AZFa, AZFb, AZFc).

Partial or complete deletions in one or more of these regions cause very low or no sperm production


INOGene Y-Microdeletion Kit includes 8 STS regions, SRY and ZFX/Y regions, including AZF-a, b and c regions.

The INOGene Y-Microdeletion Kit works on the basis of melting curve analysis using SYBR Green dye via Real-time PCR instrument.


Whole blood samples collected in EDTA tubes can be used in RT-PCR analysis after DNA extraction.

 

The kit components are shown in the table below.

 

 

Reagents Reagents Content 50 Reactions
Reaction Mix 1 SY84, SY134 900  µl
Reaction Mix 2 SY87, ZFX/Y 900  µl
Reaction Mix 3 SY254, SY127 900  µl
Reaction Mix 4 SY255, SRY 900  µl
Reaction Mix 5 SY128, SY86 900  µl
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