Covid-19 Multiplex RT-qPCR detection kit, developed at Inovia Technology laboratories, has specifically designed oligonucleotides that target gene regions that are not impacted by mutation, allowing it to detect SCoV-2 variants that have surfaced thus far, including the Omicron variant.
The probes of SARS-CoV-2 RDRP and E genes in the kit are labeled with FAM and CY5 fluorophores, respectively, while the probe of RNase P gene is labeled with HEX fluorophore as an internal control. The kit performs virus screening and extraction control in the same well in a multiplexed manner.
It is possible to control the quality of the oligonucleotides and detect possible contamination during the analysis using the reaction mix, primer-probe mix, positive control and negative control tubes provided in the kit.
Two types of packages are prepared as 100 reactions and 500 reactions and you can select the one that best suits your needs.
Furthermore, the kit's optimizations have been performed in the Inovialab GENX-4, Biorad CFX96, and Roche LC480, and results can be acquired in 47 minutes.
Kit Contents
The Kit comes in two different package sizes: 100 and 500 reactions. Table 1 lists the components of both packages.
Table 1 INOGene-SCOV-2 RT-qPCR Detection Kit components
| Reagents | 100 reactions | 100 rxn Vials | 500 reactions | 500 rxn Vials |
| Reaction Mix (Taq Polymerase,RT, RI, Buffer) | 1050 ul | 1.5 ml screw tube | 5250 ul | 5x1.5 ml screw tube |
| Primer-Probe Mix (Specially synthesized oligonucleotides for E, RDRP and Rnase P control) | 325 ul | 0.5 ml screw tube | 1550 ul | 2 ml screw tube |
| Negative Template Control (Nuclease-free water) | 400 ul | 0.5 ml screw tube | 1650 ul | 2 ml screw tube |
| Positive Control (Plasmid mix containing Covid-19 genome) | 40 ul | 0.5 ml screw tube | 200 ul | 0.5 ml screw tube |
The kit uses reporter fluorophores to target the E and RdRp genes as SARS-COV-2 controls, as well as the Rnase P gene as an internal control, as indicated in Table 2.
Table 2 Targeted genes and reporter fluorophores
|
TARGET GENE |
FLUOROPHORE |
|
E Gene |
CY5 (RED) |
|
RdRp Gene |
FAM (GREEN) |
|
Internal Control (Rnase P) |
HEX (YELLOW) |
