INOGene-Human Immunodeficiency Virus (HIV) Quantitative RT-PCR Kit
HIV is classified in the family Retroviridae, subfamily Lentivirinae, and genus Lentivirus (1).
The struc- ture of HIV follows the typical pattern of the retrovirus family, comprising a singlestranded, positive- sense ribonucleic acid (RNA) genome of about 9.7 kilobases.
INOGene-Human Immunodeficiency Virus Quantitative RT-PCR Kit assay is a one-step real time reverse transcription PCR assay in which RNA templates are first reverse-transcribed to generate complementary cDNA strands followed by DNA polymerase-mediated cDNA amplification.
INOGene-Human Immunodeficiency Virus Quantitative RT-PCR Kit utilizes external standards to gather quantitative results and includes an internal control, which controls for target isolation and amplification.
The target region is situated in gag gene region of HIV-1 genome and is 121-bases long. HIV-1 RNA concentration is noted in International Units/ml (IU/ml).
The kit components are shown in the table below;
|
Reagents |
100 reactions |
|
HIV-1 Reaction Mix |
2x1000 μl |
|
HIV-1 Enzyme Mix |
165 μl |
|
HIV-1 Internal Control |
250 μl |
|
HIV-1 Quantification Standart 1 (107 IU/ml) |
100 μl |
|
HIV-1 Quantification Standart 2 (106 IU/ml) |
100 μl |
|
HIV-1 Quantification Standart 3 (105 IU/ml) |
100 μl |
|
HIV-1 Quantification Standart 4 (104 IU/ml |
100 μl |
|
PCR Grade Water |
100 μl |
