INOGene-Hepatitis B Virus (HBV) Quantitative RT-PCR Kit
The Hepatitis B virus (HBV) with a 3.2 kbp long and partially double stranded DNA genome is a member of the Hepadnaviridae family (1).
The HBV infection can cause acute or chronic liver disease and in the chronic case, the liver infection may become life threatening by developing into cirrhosis or hepatocellular carcinoma (HCC) (2).
Its transmission is similar to but much more infectious than HIV.
INOGene-Hepatitis B Virus (HBV) Quantitative RT-PCR Kit is an in vitro nucleic acid amplification assay for quantification of Hepatitis B virus (HBV) DNA in human serum or plasma (EDTA) using viral DNa extraction kits via Real-Time PCR instrument.
The Kit is intended for use as an aid in the management of patients with chronic HBV infection undergoing anti-viral therapy to assess response to treatment in conjunction with all relevant clinical and laboratory findings.
INOGene-HBV Quantitative RT-PCR Kit is not intended for screening of blood and blood products for the presence of HBV DNA or confirmation of the diagnosis of infection with HBV.
The kit components are shown in the table below.
|
Reagents |
100 reactions |
|
HBV Mix A |
610 μl |
|
HBV Mix B |
450 μl |
|
HBV Internal Control |
250 μl |
|
HBV Quantification Standart 1 (107 IU/ml) |
100 μl |
|
HBV Quantification Standart 2 (106 IU/ml) |
100 μl |
|
HBV Quantification Standart 3 (105 IU/ml) |
100 μl |
|
HBV Quantification Standart 4 (104 IU/ml |
100 μl |
|
PCR Grade Water |
100 μl |
